Us Weekly reported that the decision was mutual and amicable, and that the two have been separated for two months. They have no children together.
Each has been divorced previously. "Men in Black 3" and "Gangster Squad" star Brolin, 45 -- who earned an Oscar nomination for portraying assassin Dan White in the 2008 historical drama "Milk" -- was married to actress Alice Adair ("Beverly Hills Cop II") from 1988 to 1994. The two have son Trevor, 24, and daughter Eden, 18. Lane, 48, Oscar-nominated for "Unfaithful" (2002), was married to the Great Neck-born French actor Christopher Lambert, also from 1988 to 1994. They have a daughter, Eleanor, 19.
Brolin is the son of Emmy Award-winning actor James Brolin ("Marcus Welby, M.D."), who married singer, actress and director Barbra Streisand in 1998, when Josh Brolin was 30. Lane's mother is October 1957 Playboy Playmate Colleen Farrington.
Lane and Brolin met in 1994, but only became a couple after being reintroduced by Streisand at a party in 2002. They became engaged in July 2003 and were married in August the following year at Brolin's 97-acre ranch in central California.
That December, Brolin was arrested at the couple's West Los Angeles home on a charge of spousal battery. He was released on $20,000 bail. His spokeswoman said at the time, "There was a misunderstanding at their home" and that the arrest was "for the lowest-end misdemeanor charge of domestic battery."