"It's great to be married, the ceremony was beautiful, and it was so special to be surrounded by our family and friends," the couple told People magazine Friday.
Meredith O'Sullivan Wasson, a spokeswoman for Biel, confirmed the news to The Associated Press.
The couple were wed Friday before family and close friends at the Borgo Egnazia resort in the southern Italian city of Puglia, E! Online reported.
Among those attending the nuptials were "Saturday Night Live's" Andy Samberg, former 'N Sync member Chris Kirkpatrick, music producer Timbaland, and Biel's "7th Heaven" co-star Beverley Mitchell, according to E! Online.
Biel and Timberlake became engaged in late December after a romance that began in January 2007 but was interrupted by a three-month split in 2011.
Timberlake proposed to the "Total Recall" actress with a 6-carat diamond ring. "I think it's important to remember that you go into something like marriage knowing that you don't know very much about it at all," he said during a recent news conference with the Hollywood Foreign Press. "But I do look at the marriage of my mother and stepdad, and what makes it work for them is that it's a team effort."