A substitute teacher at a Port Washington middle school was arrested Thursday, and accused of sexually abusing two girls in his class, village police said.

The incident between James Servin, 51, and the two students occurred in the classroom during a school day in late January at Carrie Palmer Weber Middle School, which educates sixth to eighth graders, said police Chief James Salerno.

Servin, of West 24th Street, was expected to be arraigned Friday at First District Court in Hempstead on two counts of endangering the welfare of a child, two of forcible touching and two of second-degree sexual abuse, all misdemeanors.

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Salerno said police started an investigation when one of the girl’s parents made a complaint late last month. He said Servin has been a substitute teacher in the school district for six years.

In a statement, school Superintendent Kathleen Mooney said the district notified police and is cooperating in the investigation.

A district spokeswoman said Port Washington schools will no longer call Servin as a substitute.

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“The district takes these allegations very seriously as the safety and well-being of our students and staff is our top priority,” Mooney said in the statement. “Accordingly, as is our policy in situations such as this, our immediate first step was to remove the teacher from the classroom to ensure the safety and well-being of our students.”

The district and police declined to provide other details in the case, including the date of the incident. Servin’s relatives could not be immediately reached Thursday night.