Two Republican candidates for state Assembly seats on Manhattan’s East Side will run on the “Stop de Blasio” ballot line newly created by political activists, the Manhattan GOP chair said Monday.

The line aims to rally opponents of Democratic New York City Mayor Bill de Blasio and was approved Friday.

Manhattan GOP chair Adele Malpass said in a statement 1,500 registered voter signatures were collected to create the ballot position, which “will create a timely opportunity for voters to send a message prior to the 2017 municipal elections.”

A news release from local Republican officials misspelled de Blasio’s name in promoting the ballot line, calling him “deBlasio.”

De Blasio is up for re-election in 2017. A Quinnipiac University poll that surveyed voters in late July found 51 percent disapprove of his performance as mayor.

Malpass said GOP Assembly candidates Rebecca Harary and Jon Kostakopoulos will run on the “Stop de Blasio” line as well as the Republican line. She said they are supporters of charter schools, tuition tax credits and the NYPD and called them “polar opposites” of de Blasio.

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Dan Levitan, a de Blasio spokesman, in response cited the city’s low crime and high job-creation rates as well as improving housing options, graduation rates and test scores.

“New Yorkers are interested in results, not gimmicks,” Levitan said, adding of anti-de Blasio candidates, “If they want to run against success, good luck to them, but they might do better with ideas of their own about improving New York.”