On-again, off-again couple John Mayer and Jennifer Aniston are apparently off for good.
"They had some disagreements and decided to not continue to see each other," a source told People magazine, which reported an end to the high-profile relationship -- for the second time -- on its Web site yesterday.
Another source told the magazine that Mayer cooled off the romance after Aniston returned from Europe, where she'd been promoting "Marley & Me."
Reps for the duo declined to comment.
Aniston, 40, and Mayer, 31, appeared ebullient at this year's Oscars, posing arm-in-arm before she headed overseas to promote her film and he went to work in Los Angeles writing music. They reportedly met at a 2008 Oscar party.
In recent interviews, Aniston has said she doesn't believe any relationship is "perfect" or was meant to last forever.
"That's hoping for too much," she told a British magazine this week.