The actor's third wife, Camille Donatacci, filed for divorce Thursday in Los Angeles, citing irreconcilable differences. She is seeking spousal support and custody of their children, Mason, 8, and Jude, 5, according to the filing.
"Kelsey and Camille value privacy," Grammer's rep said in a statement. "They understand that private lives sometimes get played out in public but ask on this occasion that their privacy - and the privacy of their children - be respected."
The couple married in August 1997 - their children were born via a surrogate.
Grammer also has two other children - daughter Spencer, 26, with his first wife, and daughter Greer, 18, from a previous relationship.