CBS said in a statement Thursday that Bieber will play "a troubled teen who is faced with a terrible decision regarding his only brother."
He'll make his "CSI" debut on the season premiere scheduled for Sept. 23, according to The Associated Press.
The network didn't say how many episodes will include the 16-year-old pop sensation, but called his character's plot line "an emotional story that will conclude later in the season."
ABC recently announced that Bieber will also make a cameo in the second season of "Shaq Vs.," the reality competition series with Shaquille O'Neal. The NBA star will visit Bieber and join in a dance crew competition.